We obatain many enzymes from microorganisms. The enzymes that are used in industry are usually obtained from microorganisms. These include bacteria and microscopic fungi, such as yeast. The microorganisms are grown inside large vessels called fermenters.
Closer look at fermenter
- Inside the fermenter, the microorganisms are provided with everything they need to grow and reproduce, e.g.:O2, supply of nutrients, a suitable pH and temperature.
- The microorganisms make the enzymes and release them into the liquid in which they are growing.
- The liquid can then be collected from the fermenter, and the enzymes purified before use.
Closer look at fermenter
The fermenter is a large, sterile container with a stirrer, a pipe to add feedstock* (molasses* or corn-steep liquor), and air pipes to blow air into the mixture.
The
microorganisms are added and the liquid is maintained around 260C and a pH of
5-6.
The enzymes produced by the microorganisms may be extracellular or intracellular:
* Feedstock: Raw material (input) fed into a process for
conversion into something different (output).
- Extracellular enzymes are extracted from the feedstock by filtering.
- To extract intracellular enzymes the microorganisms are filtered from the feedstock, then crushed and washed with water. The enzymes are now in solution.
Explain
words:
* Molasses (syrup): Thick, dark brown, uncrystallized juice obtained from
raw sugar during the refining process.
*Corn-steep liquor:
a concentrated fluid obtained by soaking corn grains in water
(containing 0,2% SO2) for 36 — 40 hours at 46 — 50°C.
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